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KMID : 0364219930360020238
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Korean Journal of Zoology
1993 Volume.36 No. 2 p.238 ~ p.244
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Identification and Purification of Juvenile Hormone Binding Protein from nyphantria cunea Drurv
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Lee In-Hee
Kim Hak-Ryul
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Abstract
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We identified juvenile hormone binding protein (JHBP) from last instar larval hemollvnph of Hvphantria cunea using gel filtration and non-SDS PAGE. Two kinds of JHBP in hemollnnph were found at two peaks by gel filtration (Sephadex G-100) and also at Rm values of 0.13 and 0.57 by non-SDS PAGE. JHBP was partially purified using anion exchange chromatosraphv, preparative gel filteration, and preparative PAGE. Dextrin coated charcoal (DCC) binding assay was employed to monitor the location of JHBP in chromatographic profile during the purification process. Purity of JHBP was checked by silver staining of 1091 SDS-Polyacrvlamide.
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KEYWORD
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Juvenile Hormone Binding, Protein, hemolymph, Hyphantria cunea
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